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. Author manuscript; available in PMC: 2014 Jun 15.
Published in final edited form as: Mol Cell Endocrinol. 2013 Mar 21;372(0):12–22. doi: 10.1016/j.mce.2013.03.004

Figure 5. Overexpression of c-Jun inhibits proliferation of LNCaP cells.

Figure 5

(A) Effect of c-Jun overexpression on cell number of LNCaP cells. LNCaP-rtTA-c-Jun cells (wild-type or mutants) were treated with or without 100 ng/ml of doxycycline (Dox) for 5 days. Cell numbers were counted on day 0, 1, 3, and 5. The asterisk indicates a significant difference (P<0.05) when compared with the control group (Student’s t-test). Error bars, S.E. (n=3). (B) Effect of c-Jun overexpression on cell cycle progression of LNCaP cells. LNCaP-rtTA-c-Jun cells were treated with or without Dox for 3 days, followed by flow cytometry analysis of cell cycle progression. (C) Effect of c-Jun overexpression on DNA synthesis of LNCaP cells. LNCaP-rtTA-c-Jun cells were treated with or without Dox for 3 days and followed by Brdu incorporation assay. Shown are representative merged images of BrdU labeling (red) and DAPI staining (blue). Bar, 100 μm. The number indicates the percentage of Brdu incorporated cells±S.E.