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. Author manuscript; available in PMC: 2014 May 27.
Published in final edited form as: J Mol Biol. 2013 Feb 8;425(10):1612–1626. doi: 10.1016/j.jmb.2013.02.003

Fig. 6.

Fig. 6

Characterization of stoichiometry of OmpR DNA interaction. (a) SV-AUC analyses of F1-FAM DNA in the presence and absence of various concentrations of OmpR. Each plot contains 10 μM F1-FAM with 0, 1, 2.5, 5, 10 or 20 μM OmpR (plots 1–6 respectively). (b) Integrated ITC analyses of the interactions of OmpR (circles) and BeF3−-treated OmpR (diamonds) with DNA containing sequences accommodating either 1 (open symbols) or 2 (closed symbols) OmpR molecules, F1b and F1, respectively. Data sets were each fit with a model for one set of binding sites (solid lines).