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(A) Expression of Lin9 mRNA in 3.5 and 4.5 dpc blastocysts was analyzed by in situ hybridization with a DIG-labeled Lin9 probe. As a control, Oct4 mRNA was analyzed. (B) Nuclear lysates from ESCs were immunoprecipitated with a LIN9 antibody or with nonspecific IgG as a control. Co-precipitated proteins were detected with the antibodies indicated. Input (5%) was also loaded on the gel for comparison.
