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. 2013 May 7;8(5):e62882. doi: 10.1371/journal.pone.0062882

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© 2013 Esterlechner et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Alkaline-phosphatase (AP) staining of control cells and LIN9 depleted cells. Scale bar: 200 µM (B) Expression of pluripotency markers Oct4 and Sox2 was analyzed in control-depleted cells and LIN9 depleted cells by RT-qPCR. (C) The cell cycle profile of LIN9 depleted ESCs and of control cells was analyzed by flow cytometry.