Figure 8. Western blot analysis of Skp2 and p27 protein levels in AtT-20 cells under lycopene (Lycop) or beta-carotene (β-carot) stimulation.

Treatment with Lycop or β-carot at 5 and 10 µM for 48 h did not change the Skp2 levels significantly, although a trend decrease was observed (A); after 96 h, Skp2 levels in Lycop 10 µM were decreased (C). When the cells were treated with carotenoids at 5 and 10 µM for 48 h, p27 protein levels increased significantly in β-carot 10 µM (B); after 96 h of treatment, both concentrations of carotenoids decreased p27 protein levels (D). (*p<0.05, **p<0.01, ***p<0.001).