Skip to main content
. 2013 May 7;8(5):e62745. doi: 10.1371/journal.pone.0062745

Figure 2. Ammonium-dependent growth of AmtB and Mep2 His variants.

Figure 2

A, Growth of S. cerevisae strains after 5 days at 30°C on YNB medium pH 6.0 containing 3 mM NH4Cl. WT - S. cerevisiae strain 23344c, Δ-S. cerevisiae strain 31019b lacking all three mep genes, AmtB - strain 31019b expressing wild-type E. coli AmtB, or with mutated versions of amtB changing the residues indicated – final four lanes. B, as for A, but with S. cerevisiae strain 31019b now expressing Mep2 or mutated versions of mep2 changing the residues indicated – final three lanes. C, Methylammonium uptake activity of wild-type E. coli AmtB and variants, measured in E. coli (Data taken from Ref [23], [44]).