Table 1. Strains and plasmids.
Relevant Genotype | Reference | |
Strain | ||
S. cerevisiae | ||
23344c | MATα ura3 | [8] |
31019b | MATα ura3 mep1Δ mep2 Δ::LEU2 mep3 Δ::KanMX2 | [8] |
Plasmid | ||
pDR195 | E. coli-S. cerevisiae shuttle vector | [45] |
pTF14 | E. coli amtB in pDR195 | This work |
pTF17 | E. coli amtB H168A in pDR195 | This work |
pTF18 | E. coli amtB F215A in pDR195 | This work |
pTF19 | E. coli amtB H318A in pDR195 | This work |
pTF20 | E. coli amtB H168A, H318A in pDR195 | This work |
pTF28 | S. cerevisiae mep2 in pDR195 | This work |
pTF29 | S. cerevisiae mep2 H194A in pDR195 | This work |
pTF31 | S. cerevisiae mep2 H348A in pDR195 | This work |
pTF32 | S. cerevisiae mep2 H194A H3489A in pDR195 | This work |