Table 1. Strains and plasmids.

	Relevant Genotype	Reference
Strain
S. cerevisiae
23344c	MATα ura3	[8]
31019b	MATα ura3 mep1Δ mep2 Δ::LEU2 mep3 Δ::KanMX2	[8]
Plasmid
pDR195	E. coli-S. cerevisiae shuttle vector	[45]
pTF14	E. coli amtB in pDR195	This work
pTF17	E. coli amtB H168A in pDR195	This work
pTF18	E. coli amtB F215A in pDR195	This work
pTF19	E. coli amtB H318A in pDR195	This work
pTF20	E. coli amtB H168A, H318A in pDR195	This work
pTF28	S. cerevisiae mep2 in pDR195	This work
pTF29	S. cerevisiae mep2 H194A in pDR195	This work
pTF31	S. cerevisiae mep2 H348A in pDR195	This work
pTF32	S. cerevisiae mep2 H194A H3489A in pDR195	This work