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. 2013 May 7;8(5):e62822. doi: 10.1371/journal.pone.0062822

Figure 3. HDACi sensitivity is decreased by over-expression of BCL-2 or BCL-XL and increased by over-expression of BIM.

Figure 3

Creation of four independent cell lines (SUDHL2 cells over-expressing BCL-2, SUDHL4 cells over-expressing BCL-XL, Farage cells over-expressing BCL-XL, and Farage cells over-expressing BIM) was confirmed by Western blotting for BCL-2, BCL-XL, and BIM (A–D) and compared to empty vector (–) transduced cells. β-tubulin was used as a normalizing control. (A) SUDHL2-BCL-2, (B) SUDHL4-BCL-XL, (C) Farage-BCL-XL, and (D) Farage-BIM cell lines were treated with either 300 nM TSA or 3 µM vorinostat for 24 h. Whole-cell extracts were made and Western blotting for PARP cleavage and β-tubulin (as a normalizing control) was performed. All cells over-expressing the protein of interest were compared to empty vector (–) transduced control cells.