Figure 5. Levels of total and phosphorylated Akt, AMPK, ACC and GSK-3β in myocardium from low fat (LF) or high fat (HF)-fed C57 mice with or without TUDCA treatment (300 mg/kg for 15 days).

A: Representative gel blots of Akt, pAkt, AMPK, pAMPK, ACC, pACC, GSK-3β, pGSK-3β and α-tubulin (loading control) using specific antibodies. B: pAkt-to-Akt ratio; C: pAMPK-to-AMPK ratio; D: pACC-to-ACC ratio; and E: pGSK-3β-to-GSK-3β ratio; Meanυ±SEM; sample sizes are denoted in the bar graphs; *p<0.05 (two-way ANOVA).