Table 2. Illumina Infinium HumanMethylation450 BeadChip array data.
Gene | Chromosome location | CpG of interest | Gene region | CpG island? | DNA methylation (β value) | Δ β | P-value | |
Control | EOPET | |||||||
NR3C1 | ch5 (q31.3) | cg14558428 | exon 1D promoter | Y | 0.14±0.08 | 0.23±0.12 | 0.09 | 0.01 |
NR3C1 | ch5 (q31.3) | cg24026230 | exon 1D promoter | Y | 0.11±0.06 | 0.19±0.13 | 0.08 | 0.02 |
CRH | ch8 (q13.1) | cg23409074 | exon 1 (5′ UTR) | N | 0.37±0.12 | 0.29±0.06 | −0.08 | 0.03 |
CRHBP | ch5 (q13.3) | cg13777717 | intron 3 | Y | 0.40±0.08 | 0.48±0.07 | 0.08 | 0.001 |
CYP11A1 | ch15 (q24.1) | cg06285340 | exon 1 | N | 0.54±0.03 | 0.42±0.04 | −0.12 | <0.0001 |
HSD3B1 | ch1 (p12) | cg16175792 | exon 2 | N | 0.82±0.02 | 0.69±0.05 | −0.13 | <0.0001 |
TEAD3 | ch6 (p21.31) | cg10893014 | intron 1 | N | 0.60±0.06 | 0.39±0.10 | −0.21 | <0.0001 |
CYP19 | ch15 (p21.2) | cg15329467 | placental-specific exon 1.1 promoter | N | 0.56±0.05 | 0.46±0.05 | −0.10 | <0.0001 |
The Illumina CpG of interest, gene location and CpG island (defined as length >200 base pairs, 50% GC content and >0.6 CG ratio) association is provided. Mean ± SD methylation β values and P-values are given for each gene for control (n = 19) and early onset pre-eclampsia (EOPET; n = 19) placental samples. NR3C1: nuclear receptor subfamily 3, group C, member 1, CRHBP: corticotropin releasing hormone (CRH) binding protein: HSD3B1∶3β-hydroxy-delta-5-steroid dehydrogenase type 1, TEAD3: TEA domain family member 3.