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Induction of cardiomyocyte-like cells in infarct hearts by a single polycistronic vector of Gata4/Mef2c/Tbx5. (A) We developed a polycistronic retrovirus expressing GMT at near equimolar levels from the same promoter using “self-cleaving” 2A peptides (3F2A). Schematic representation of the 3F2A retrovirus containing Gata4, Mef2c and Tbx5 (GMT).14) (B) 3F2A retrovirus induced α-actinin expression and cross striations. White boxes are enlarged in the insets.14)
