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. 2013 May 7;104(9):1933–1939. doi: 10.1016/j.bpj.2013.02.053

Figure 4.

Figure 4

Adsorption of R9C depends strongly on the content of the negatively charged lipid (DOBMP). The graph shows the effect of 1 μM cis side R9C addition on the single-channel gramicidin A conductance, recorded at V = +100 mV in the specified membranes. In the absence of R9C the channel conductance increases proportionally to the DOBMP content in DOBMP/DOPC lipid mixture (open circles); however, addition of R9C reduces the channel conductance even beyond its value in the neutral lipid (solid diamonds). The inset gives the ratio of the channel conductances in the absence and presence of R9C. Membrane buffer contained 150 mM CsCl, 5 mM HEPES, pH 7.4.