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. 2013 May 7;104(9):1933–1939. doi: 10.1016/j.bpj.2013.02.053

Figure 5.

Figure 5

The adsorption of R9C does not affect lipid mixing in the asymmetric DOBMP/DOPE (4:1) cis//DOTAP trans bilayer, as probed with gramicidin A channel conductance (R9C was applied from the cis side). In the asymmetric membrane the current through a single channel is highly asymmetric in voltage (open circles, see also (7)). Application of 1 μM R9C produces a strong effect on the channel current at the applied voltages positive from the DOBMP/DOPE leaflet side (solid squares). However, the adsorbed R9C can be effectively removed from the membrane surface by applying polyanionic dextran sulfate (50 μM) so that the initial asymmetric current-voltage curve is restored (open diamonds). Membrane buffer contained 150 mM CsCl, 5 mM HEPES, pH 7.4.