Table 1.
Constructs | Unloaded Velocitya,c |
Run lengthb,c |
Stall forcea,d |
Three-state modelb,d |
|||||
---|---|---|---|---|---|---|---|---|---|
k1e |
k−1e |
k20 |
k3 |
δ2 |
vmax |
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(nm/s) | (nm) | (pN) | (μM−s)−1 | (s−1) | (s−1) | (s−1) | (nm) | (nm/s) | |
WT | 671 ± 21 | 1370 ± 287 | 4.92 ± 0.08 | 2 | 120 | 12,900 ± 6540 | 76.86 ± 1.88 | 5.50 ± 0.51 | 630 ± 15 |
Kin-1CS | 579 ± 18 | 982 ± 129 | 3.89 ± 0.05 | 2 | 120 | 4260 ± 2060 | 72.74 ± 2.45 | 5.65 ± 0.60 | 597 ± 20 |
Kin-1NL | 501 ± 26 | 225 ± 59 | 2.95 ± 0.05 | 2 | 120 | 3640 ± 2200 | 63.21 ± 2.19 | 7.35 ± 0.99 | 518 ± 21 |
Kin-1L13 | 440 ± 38 | 263 ± 89 | 2.79 ± 0.06 | 2 | 120 | 2120 ± 1410 | 56.43 ± 2.97 | 7.27 ± 1.18 | 463 ± 24 |
Kin-1CS·NL | 522 ± 28 | 482 ± 83 | 3.15 ± 0.04 | 2 | 120 | 9440 ± 6320 | 65.36 ± 2.19 | 8.69 ± 1.10 | 536 ± 18 |
Kin-1CS·NL·L13 | 529 ± 33 | 258 ± 27 | 2.78 ± 0.03 | 2 | 120 | 1190 ± 573 | 69.42 ± 3.98 | 6.22 ± 0.85 | 569 ± 33 |
Values are mean ± standard error.
Values are fitted value ± standard deviation.
n = 35, 34, 45, 24, 38, 50.
n = 185, 364, 292, 153, 422, 585, for each of the six constructs listed top to bottom in the left column of the table.
Globally fit, values from Valentine and Gilbert (61).