Figure 8.

NTM inhibits glucose‐induced nuclear translocation of ChREBP in HepG2 cells. HepG2 cells were starved for 24 hours in DMEM containing 5.5 mmol/L glucose then refed with 25 mmol/L glucose+100 nmol/L insulin±30 μmol/L cSN50.1 for 24 hours. Nuclear extracts were immunoblotted with antibodies to ChREBP and Lamin A/C (loading control). Samples shown are from the same membrane. Values from glucose‐induced samples without cSN50.1 treatment were set to 100% and percent values for peptide‐treated samples calculated. The difference represents percent inhibition of nuclear translocation by cSN50.1, shown as the mean+SEM of 3 independent experiments (*P<0.05 by Student's t test). NTM indicates nuclear transport modifier; ChREBP, carbohydrate responsive element‐binding protein.