Table 1.

Amino Acid Sequences of NTM Peptides^* and Peptide Modules

	SSHR	NLS
SN50	AAVALLPAVLLALLAP	VQRKRQKLMP
cSN50.1	AAVALLPAVLLALLAP	CVQRKRQKLMPC
N50		VQRKRQKLMP
cN50.1		CVQRKRQKLMPC
SSHR‐1*	AAVALLP
SSHR‐2*	AVLLALLAP

Hydrophobic regions of the SSHR domain are distinguished from the cluster of basic amino acids (NLS). Combinations of listed peptides were tested in pull‐down assays. Because of limited solubility of SSHR‐derived peptides in aqueous solution, a solubility tag was added to the amino terminus of these peptides. For pull‐down experiments, all peptides were labeled with biotin. NTM indicates nuclear transport modifier; SSHR, signal sequence hydrophobic region; NLS, nuclear localization sequence.

^*N‐biotinylated versions of these peptides were used in binding and competition assays.

^*Solubility tag added to the N‐terminus of these peptides.