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. Author manuscript; available in PMC: 2014 May 15.
Published in final edited form as: J Immunol. 2013 Apr 12;190(10):5216–5225. doi: 10.4049/jimmunol.1300097

Figure 2. Reconstitution of DMXAA induced IFNβ signaling in STING expressing HEK293 cells.

Figure 2

(A) 293T cells were transfected with either empty vector or mSTING in the presence of an IFN-β luciferase reporter gene (left panel) or a multimerized PRDIII-I reporter gene (right panel) and transfected cells stimulated with DMXAA from 10–100 μg/ml for 18hours and luciferase activity was measured. Data are presented as the mean ± s.e.m of one experiment representative of three experiments. * indicates p <0.05 for the comparison of the highest dose of DMXAA relative to vector control. (B) 293T cells transfected as above were stimulated with cyclic-di-GMP (10μg/ml) or with increasing amounts of DMXAA as in A and endogenous TBK1 activity was analyzed by in vitro kinase assay.