Table 1.
Plasmids used in this study
| Plasmid | Description | Reference |
| pRM2 | Yeast expression plasmid (CEN, URA3) encoding human GLUT4 from the CUP1 promoter | (Lamb et al., 2010) |
| pRM3 | Yeast expression plasmid (CEN, URA3) encoding human GLUT4 with lysines 109, 242, 245, 261, 264, 266, 495 mutated to arginine residues (G4-7K/R) from the CUP1 promoter | (Lamb et al., 2010) |
| pRM17 | Yeast expression plasmid encoding human GLUT4 with a single cytosolic lysine at position 242 from the CUP1 promoter (derived from pRM3) | This study |
| pRM18 | Yeast expression plasmid encoding human GLUT4 with a single cytosolic lysine at position 261 from the CUP1 promoter (derived from pRM3) | This study |
| pRM19 | Yeast expression plasmid encoding human GLUT4 with a single cytosolic lysine at position 109 from the CUP1 promoter (derived from pRM3) | This study |
| pRM20 | Yeast expression plasmid encoding human GLUT4 with a single cytosolic lysine at position 245 from the CUP1 promoter (derived from pRM3) | This study |
| pRM21 | Yeast expression plasmid encoding human GLUT4 with a single cytosolic lysine at position 264 from the CUP1 promoter (derived from pRM3) | This study |
| pRM22 | Yeast expression plasmid encoding human GLUT4 with a single cytosolic lysine at position 266 from the CUP1 promoter (derived from pRM3) | This study |
| pRM23 | Yeast expression plasmid encoding human GLUT4 with a single cytosolic lysine at position 495 from the CUP1 promoter (derived from pRM3) | This study |
| pRM24 | Yeast expression plasmid (CEN, URA3) encoding G4-7K/R carrying an HA-tag fused in-frame to ubiquitin from the CUP1 promoter | This study |
| pCAL4 | Yeast expression plasmid (CEN, URA3) encoding HA-tagged human GLUT4 from the CUP1 promoter | This study |
| pCAL5 | Yeast expression plasmid (CEN, URA3) encoding HA-tagged G4-7K/R from the CUP1 promoter | This study |
| pHA-GLUT4 | Retroviral expression plasmid encoding HA-tagged human GLUT4. | (Shewan et al., 2000) |
| pHA-TAIL | Retroviral expression plasmid encoding HA-tagged human GLUT4 with the C-terminal 12 amino acid residues replaced with the corresponding sequence from human GLUT3. | (Shewan et al., 2000) |
| pHA-G4/Gap1 | Retroviral expression plasmid encoding HA-tagged human GLUT4 with the entire C-terminal cytosolic tail (residues 470–509) replaced with the analogous region of the yeast protein Gap1 (residues 547–602). | This study |