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. 2013 May 3;8:1595–1607. doi: 10.2147/IJN.S43244

Figure 10.

Figure 10

Extracellular matrix mineralization mediated by anti-miR-138 and miR-148b. Transfected mesenchymal stem cells were stained using Alizarin red after 14 and 21 days of culture in osteogenic medium. (A and A`) Reverse transfection formulation of anti-miR-138, (B and B`) reverse transfection formulation of miR-148b, (C and C`) reverse transfection formulation of anti-miR-control, (D and D`) reverse transfection formulation of anti-miR-control and (E and E`) blank control tissue culture plate. The black arrows in (D and E) indicate tiny mineralized dots. Scale bar in micrographs represents 100 μm. (F and F`) Quantitative colorimetric results.

Notes: **P < 0.01 and ***P < 0.001 versus blank control tissue culture plate; ###P < 0.001 versus miR-control group; @@@P < 0.001 versus anti-miR-control group; ♦♦♦P < 0.001 versus miR-148b group.