Figure 7.

Osteogenesis-related gene expression mediated by reverse transfection of anti-miR-138 and miR-148b, ie, relative expression of alkaline phosphatase, bone morphogenetic protein-2, collagen type 1 α1, osteocalcin, osterix, and runt-related transcription factor 2, was assessed by real time reverse transcription polymerase chain reaction for mesenchymal stem cells transfected with lyophilized formulations carrying anti-miR-138, miR-148b, anti-miR-control, and miR-control after culturing in osteogenic medium for 7 days and 14 days.

Notes: The tissue culture plate is set as the blank control. *P < 0.05; **P < 0.01; and ***P < 0.001 versus blank control tissue culture plate; ^@@P < 0.01 and ^@@@P < 0.001 versus reverse transfection formulation of anti-miR-control; ^#P < 0.05; ^##P < 0.01; and ^###P < 0.001 versus reverse transfection formulation of miR-control; ^♦P < 0.05, ^♦♦P < 0.01, and ^♦♦♦P < 0.001 versus reverse transfection formulation of miR-148b.