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. 2013 Mar;19(3):496–499. doi: 10.3201/eid1903.121017

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Western blot reaction of serum samples from patients HuC1 and HuC2 from Italy showing allergic reaction against Anisakis pegreffii antigens and allergens. M indicates molecular marker; arrow indicates the reaction at 24 kDa (Ani s1). IgE determination was performed with alkaline phosphatase conjugates obtained from goat anti-human IgE. Antigen-antibody binding was visualized by the alkaline phosphatase 5-bromo-4-chloro-3-indolyl phosphate p-nitroblue tetrazolium chloride system until bands appeared. Human serum specimens negative for A. pegreffii were used as controls. HC1 and HC2 represent patient identification numbers HuC1 and HuC2.