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. 2013 Mar;19(3):496–499. doi: 10.3201/eid1903.121017

Table. Clinical features of patients who had gastroallergic anisakiasis associated with Anisakis pegreffii infection, Italy*.

Code Home area of patient, province (region) Location of nematode Time since raw seafood ingested Clinical features Whole larval nematode or fragment, suspension, condition of specimen DNA extraction method Total IgE, IgE-As†
HuC1 Benevento (Campania) Submucosal layer of gastric wall 2 d Epigastric pain, edema of oral mucosa Whole, 99% ethanol, very well conserved CTAB 1,479, >100
HuC2 Pescara (Abruzzo) Gastric mucosa 24 h Epigastric pain, urticaria, generalized edema Whole, 99% ethanol, well conserved CTAB 2,180, >100
HuC3 Pescara (Abruzzo) Lumen of stomach 24 h Epigastric pain Fragment, 99% ethanol, well conserved CTAB 4,727, >100.0
HuC4 Pescara (Abruzzo) Lumen of stomach 1 d Epigastric pain, digestive symptoms Fragment, 99% ethanol, well conserved CTAB 511, 21.2
HuC5 Pescara (Abruzzo) Lumen of stomach 1 d Epigastric pain Fragment 99% ethanol, well conserved CTAB Serum not available
HuC6 Rome (Latium) Lumen of stomach 1 d Epigastralgia, urticaria Fragment 99% ethanol, well conserved CTAB Serum not available
HuC7 Pisa (Tuscany) Lumen of stomach 2 d Epigastric pain, vomiting Fragment spoiled, 10% formalin, poorly conserved Maxwell 16 Promega‡ 2,062, 89.3
HuC8 Naples (Campania) Lumen of stomach 2–3 h Epigastralgia Fragment spoiled, 10% formalin, poorly conserved Maxwell 16 Promega Serum not available

*Patients are indicated by their codes, HuC1–HuC8; all patients reported having eaten marinated anchovies before onset of illness. CTAB, cetyltrimethylammonium bromide (8).
† Determined by ImmunoCAP ISAC diagnostic test (Phadia, Uppsala, Sweden).
‡Maxwell 16 Instrument System (Promega, Madison, WI, USA)