Table 1. Overview of bats tested for 2c betacoronaviruses, Ghana and Europe.

Area, bat species	No. bats tested (no. [%] positive)*	Age, juvenile/adult†	Sex, F/M‡	Location§ (no. tested/no. positive)
Ghana
Coleura afra	108 (0)	2/105	46/59	a, b, e
Hipposideros abae	604 (0)	55/548	207/341	a, b, d, f
H. cf. gigas	28 (0)	7/19	8/11	a, b, d
H. fuliginosus	1 (0)	1/0	Unknown	c
H. jonesi	31 (0)	6/25	1/24	c, d
H. cf. ruber	3,763 (0)	674/3,078	1,109/1,969	a, b, c, d, f, g
Nycteris cf. gambiensis	185 (46 [24.9])	22/161¶	79/82	a# (5/2), b# (65/15), d# (104/29), f (1/0)
Rhinolophus alcyone	4 (0)	2/2	1/1	c
R. landeri	13 (0)	3/10	2/8	b, d, f
Taphozous perforatus	21 (0)	3/18	0/18	e
Total	4,758 (46 [1.0])
Europe
Pipistrellus kuhlii	7 (0)	Unknown	3/3	l
P. nathusii	82 (30 [36.6])	15/65	38/43	j (2/0), k# (74/29), l# (6/1)
P. pipistrellus	42 (1 [2.4])	17/25	19/21	i (29/0), k# (7/1), h (6/0)
P. pygmaeus	141 (9 [6.4])	11/127	83/55	j (44/0), k# (91/9), l (6/0)
Total	272 (40 [14.7])

*The real-time reverse transcription PCR (Ghana) used oligonucleotides 2c-rtF, 5′-GCACTGTTGCTGGTGTCTCTATTCT-3′, 2crtR, 5′-GCCTCTAGTGGCAGCCATACTT-3′ and 2c-rtP, JOE-TGACAAATCGCCAATACCATCAAAAGATGC-BHQ1 and the Pan2c-heminested assay (Europe) used oligonucleotides Pan2cRdRP-R, 5′-GCATWGCNCWGTCACACTTAGG-3′; Pan2cRdRP-Rnest, 5′-CACTTAGGRTARTCCCAWCCCA-3′; and Pan2cRdRp-FWD, 5′-TGCTATWAGTGCTAAGAATAGRGC-3′.
†Excludes bats (all coronavirus-negative) that were missing data for age.
‡ Excludes bats that were missing data for sex.
§a, Bouyem; b, Forikrom; c, Bobiri; d, Kwamang; e, Shai Hills; f, Akpafu Todzi, g, Likpe Todome; h, Province Gelderland; i, Eifel area; j, Holstein area; k,Tulcea county; l, Kiev region; GPS coordinates are shown in Figure 1.
¶For 2 animals, no data on age were available.
#Locations in which coronavirus 2c–positive bats were found.