Table 2.
Primers used for real-time PCR analysis of gene expression
| Gene | Direction | Primer sequence | Product size (bp) | GenBank accession no. |
|---|---|---|---|---|
| GAPDH | Forward | ATGTTCGTGATGGGTGTGAA | 176 | AY436595.1 |
| Reverse | CTGTCTTCGTGTGTGGCTGT | |||
| MHC-I | Forward | GAAGGAAGAGACTTCATTGCCTTGG | 196 | AB115246.1 |
| Reverse | CTCTCCTCTCCAGTACGTCCTTCC | |||
| MHC-II | Forward | CCACCTTTACCAGCTTCGAG | 229 | AY905540.1 |
| Reverse | CCGTTCTTCATCCAGGTGAT | |||
| CD40 | Forward | CTGAGAAGAGGCAGCCGATT | 132 | EU918729 |
| Reverse | TCAGCTTCCCTTTGTGGTAGGA | |||
| CD154 | Forward | CCACATGGCAGGACTGAAGAG | 110 | DQ267671 |
| Reverse | CCTTCAGCTTCCCTTTGTGGTA | |||
| IL-2 | Forward | TTCGCCAAGAGCTGACCAA | 161 | AF294323.1 |
| Reverse | AAGAGCATACAGTGGTCCCAAAG | |||
| IFN-γ | Forward | GACCTCGTGGAACTGTCAAACC | 168 | AJ012254.1 |
| Reverse | AAAGGCCGTATGATGTCATTAACA | |||
| IL-6 | Forward | CGGGCTTTTCACCTTTCAGA | 107 | AB191038 |
| Reverse | TCGTTGCCAGATGCTTTGTG |