Table 1.

Oligonucleotides used to amplify the target lig sequences

Antigen used for vaccine preparation	Plasmid and oligonucleotidea	Amino acidsb	Molecular mass (kDa)
LigAni	pAE_F, 5′-CCGCTCGAGGATATTCTTACCGTTTC-3′	629–1224	64
	pAE_R, 5′-CGGGAATTCTGGCTCCGTTTTA-3′
	pTARGET_F, 5′-ATGGATATTCTTACCGTTTC-3′
LigBni	pAE_F, 5′-CCGCTCGAGGATATTGCTGAAATT-3′	629–1112	52
	pAE_R, 5′-CGGGAATTCTCCAGATACTGAACC-3′
	pTARGET_F, 5′-ATGCTCGAGGATATTGCTGAAATT-3′
LigBrep	pAE_F, 5′-CCGCTCGAGGTGTTTATGAAGAAA-3′	1–628	72
	pAE_R, 5′-ACTCTCGAGCGTATTAGAGGAAT-3′
	pTARGET_F, 5′-ATGGTGTTTATGAAGAAAATATTTTTG-3′
LigBct1	pAE_F, 5′-CCGCTCGAGAATACAATCCTCACA-3′	1113–1501	45
	pAE_R, 5′-GGGAAGCTTTATAAAAATATGAGA-3′
	pTARGET_F, 5′-ATGAATACAATCCTCACA-3′
LigBct2	pAE_F, 5′-CCGCTCGAGGCAGGTTCAAGACC-3′	1502–1891	45
	pAE_R, 5′-GGGAAGCTTTTATTGATTCTGTT-3′
	pTARGET_F, 5′-ATGGCAGGTTCAAGACC-3′

^aIn the oligonucleotide sequences, the underlined letters denote the restriction sites.

^bAmino acid coordinates in the LigA and LigB proteins of L. interrogans serovar Copenhageni strain FIOCRUZ L1-130.