Fig 2.

Bacterial recovery from the lung during the acute phase of infection with S. pneumoniae ST 3. Immunized mice were inoculated with a dose of 5 × 10⁴ CFU and euthanized at 24 h p.i. or inoculated with a dose of 5 × 10⁴ CFU or 1 × 10⁵ CFU and euthanized 48 h p.i. Lung homogenates were serially diluted and plated overnight to determine bacterial burden. Log CFU mean values and standard errors of the means are shown, with each point representing one mouse. (A) Lung bacterial recovery at 24 h p.i. An asterisk denotes a P value of <0.05 as determined by a Student's t test between vehicle and coimmunized mice (n = 8 per group). (B) Lung bacterial recovery at 48 h p.i. from mice immunized with vehicle (n = 16), PPS3 (n = 12), LPS (n = 4), and PPS3+LPS (n = 21). Asterisks denote P values of <0.05 (*) and <0.001 (**) as determined by one-way ANOVA with Tukey's multiple comparison posthoc test in comparison to vehicle-immunized infected controls. Data are shown combined from two separate in vivo infection experiments (A) or four independent infections (B).