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. 2013 May;20(5):639–650. doi: 10.1128/CVI.00593-12

Table 1.

Primer pair details for Q-PCR

Primer target Accession no.a Primer sequence Melting temp (°C) Product size (bp)
CCL2 NM 011333 5′-AGGTCCCTGTCATGCTTCTG 60 248
3′-TCTGGACCCATTCCTTCTTG
IL-1β NM 010548 5′-GCCCATCCTCTGTGACTCAT 63 229
3′-AGGCCACAGGTATTTTGTCG
IL-6 NM 031168 5′-TTCCATCCAGTTGCCTTCTT 60 493
3′-GAGCATTGGAAATTGGGGTA
IL-10 NM 008361 5′-ACGGAAACAACTCCTTGGAA 60 485
3′-AACTGGCCACAGTTTTCAGG
LBP NM 008489 5′-GTGGCTGCTGAATCTCTTCC 55 249
3′-GAGCGGTGATTCCGATTAAA
Saa1 NM 009117 5′-GAGGACATGAGGACACCATTGC 63 196
3′-CCAGAGAGCATCTTCAGTGTTCC
TNF-α NM 013693 5′-AGCCCCCAGTCTGTATCCTT 63 211
3′-CTCCCTTTGCAGAACTCAGG
18s rRNA M35283 5′-AATGGTGCTACCGGTCATTC 60 192
3′-ACCTCTCTTACCCGCTCTCC
a

Accession numbers are for Mus musculus.