Fig 3.

Western blots demonstrating that the breadth of the antibody responses induced by immunization were similar in both groups of immunized animals. An immunoblot was exposed to film for 40 s for both immunized groups (A) and for 1 min for the unlinked group in order to more clearly demonstrate bands (B). Antibodies in both groups were predominantly directed toward Msp2 (36 kDa) and Msp3 (74 kDa), which are known immunodominant A. marginale proteins (arrowheads). The unlinked group received cross-linked outer membrane protein complexes in which the cross-links had been reduced. The linked group received intact cross-linked outer membrane protein complexes. Animal numbers are listed across the top. No specific antibodies were detected in animals prior to the immunization shown for animal 124 (124P) or in animals inoculated with (C) adjuvant alone. The serum dilution was 1:100 for all groups.