Table 3.
Lymphoproliferative and IFN-γ release responses in supernatants of antigen-stimulated PBMC cultures from leprosy patients with type 1 leprosy reactionsa
| PBMC response | Patient no. |
||||
|---|---|---|---|---|---|
| 17 | 18 | 19 | 20 | 21 | |
| Mean Δcpm ± SD (SI) for responses to: | |||||
| Peptide A | 951 ± 856 (1.3) | 11,569 ± 1875 (14.4) | 1,003 ± 253 (1.8) | 885 ± 104 (2.3) | 3,373 ± 185 (6.1) |
| Peptide B | 385 ± 230 (0.98) | 12,034 ± 2012 (10.5) | 448 ± 92 (1.8) | 808 ± 85 (2.1) | 2,297 ± 201 (5.1) |
| Peptide C | 2,097 ± 245 (1.8) | 11,930 ± 1252 (10.4) | 1,540 ± 155 (2.0) | 1,694 ± 235 (4.4) | 5,136 ± 127 (10.2) |
| Peptide D | 1,398 ± 230 (1.6) | 10,091 ± 1254 (8.9) | 492 ± 88 (1.5) | 847 ± 35 (2.2) | 6,672 ± 327 (12) |
| Peptide E | 17,852 ± 2130 (7.4) | 17,471 ± 1275 (12.8) | 12,182 ± 2340 (9.2) | 14,630 ± 1824 (38) | 3,515 ± 97 (7.3) |
| Peptide F | 1,147 ± 127 (1.5) | 3,936 ± 203 (4.1) | 393 ± 54 (1.5) | 809 ± 203 (2.1) | 6,370 ± 1450 (12.3) |
| Lsr2 | 1,851 ± 230 (2.5) | 17,010 ± 203 (12.4) | 1,950 ± 156 (2.3) | 1,386 ± 167 (3.6) | 5,824 ± 203 (11.4) |
| M. leprae | 820 ± 102 (2.3) | 2,910 ± 128 (2.4) | 722 ± 56 (2.3) | 848 ± 150 (2.2) | 6,777 ± 1295 (13.1) |
| Mean IFN-γ ± SD (pg/ml) for responses to: | |||||
| Peptide A | 205 ± 7.5 | 540 ± 13.0 | 102 ± 6.5 | 175 ± 1.5 | 562 ± 10.4 |
| Peptide B | 75 ± 2.2 | 735 ± 12.0 | 182 ± 6.4 | 315 ± 8.5 | 523 ± 12.5 |
| Peptide C | 128 ± 7.5 | 450 ± 12.5 | 205 ± 3.5 | 507 ± 5.5 | 645 ± 11.0 |
| Peptide D | 82 ± 2.5 | 452 ± 12.0 | 267 ± 7.5 | 312 ± 5.5 | 726 ± 10.2 |
| Peptide E | 746 ± 8.4 | 620 ± 13.2 | 820 ± 10.6 | 1982 ± 11.2 | 567 ± 10.2 |
| Peptide F | 205 ± 7.5 | 180 ± 6.2 | 120 ± 2.5 | 275 ± 8.5 | 845 ± 13.1 |
| Lsr2 | 215 ± 2.5 | 825 ± 2.7 | 218 ± 3.8 | 259 ± 9.7 | 925 ± 12.6 |
| M. leprae | 102 ± 4.5 | 230 ± 5.7 | 245 ± 6.5 | 205 ± 8.6 | 1,024 ± 13.4 |
a
Peptides A through F are peptides of Lsr2, M. leprae, and heat-killed M. leprae. Unstimulated culture supernatants showed mean IFN-γ levels ranging from 8 to 10 pg/ml. Numbers in parentheses indicate the stimulation index, as described in Materials and Methods.