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The role of Vps75 and the carboxyl terminus of Asf1 in H3K56 acetylation in vivo. (A and C to F) WCE were prepared from the indicated strains, separated by 15% SDS-PAGE, transferred onto nitrocellulose, and immunoblotted with indicated antibodies. (B) FACS analysis indicates no significant change in cell cycle profile of the rtt109Δ or rtt109 Δvps75Δ strain expressing either the full-length 12MYC-RTT109 or the 12MYC-RTT109(1–424) mutant.
