Fig 6.

Lysine 290 of Rtt109 is important for in vivo H3K56ac and H3K9ac. (A) 12MYC-RTT109K290Q rtt109Δ and 12MYC-RTT109K290R rtt109Δ strains show significantly decreased levels of H3K9ac but not H3K56ac in vivo. Western blotting was performed as described in the legend of Fig. 5A. (B) 12Myc-Rtt109K290Q and 12Myc-Rtt109K290R are able to interact with Vps75-TAP. Immunoprecipitations were done as described in the legend of Fig. 3A. (C) 12MYC-RTT109(1–424)K290Q rtt109Δ and 12MYC-RTT109(1–424)K290R rtt109Δ strains have significantly decreased levels of H3K56ac in vivo. WCE were prepared from the indicated strains, separated by 15% SDS-PAGE, transferred onto nitrocellulose, and immunoblotted with anti-Myc, anti-H3K56ac, and anti-H3.