Fig 3.

Time course experiments of sterol uptake into C. albicans, S. cerevisiae, and C. glabrata. Cholesterol (A, B, and C) and ergosterol (D, E, and F) uptake by C. albicans (circles), S. cerevisiae (squares), and C. glabrata (triangles) under normoxic (A, C, D, and F) and microaerophilic (B and E) conditions. Parental strains are represented by solid symbols and UPC2-null mutants by open symbols. Strains were grown for 5 days in CSM complete supplemented with uridine, Tween 80, and a mix of cold and radioactively labeled cholesterol-ergosterol. Samples were analyzed every 24 h. The graphs represent average of three biological replicates with standard deviation. (G and H) Cholesterol uptake in three fungal species presented as a percentage of all available cholesterol taken up from the medium under normoxic (G) and microaerophilic (H) conditions. The graphs in panels G and H were created from data presented also in panels A and B.