Fig 4.

TLC separation of radioactively labeled cholesterol and ergosterol associated with the cells of C. albicans, S. cerevisiae, and C. glabrata. Set of parental strains of the three fungal species were compared to each other under normoxic (2 and 5 days) and microaerophilic (2 days) conditions in the presence of radioactively labeled cholesterol (A) or ergosterol (B). AUS1/PDR11 mutants were compared to their parents only in the presence of radioactively labeled cholesterol (C). Total cell lipids were extracted from the cells and separated by TLC. The samples represent the amount of cells grown in equal volume of culture medium. Chol, cholesterol; CE, cholesteryl ester; Erg, ergosterol; EE, ergosteryl ester.