Fig 5.

Uptake of fluorescently labeled cholesterol from agar medium. Cell cultures of C. albicans, S. cerevisiae, and C. glabrata strains spotted on CSM complete supplemented with uridine and cholesteryl-BODIPY (fluorescently labeled cholesterol) were incubated for 2 days under normoxic and microaerophilic conditions and scanned. The zone of clearance (white area) around colonies is indicative of decreased local concentration of cholesteryl-BODIPY and thus active uptake of this sterol. S. cerevisiae parental strains differ for the UPC2 mutants (W303-1a) and AUS1/PDR11 mutant (BY4742) and thus are presented both separately with the appropriate mutants.