Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 May;12(5):684–696. doi: 10.1128/EC.00017-13

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2013, American Society for Microbiology. All Rights Reserved.

PMC Copyright notice

Fig 7 — Rapid shifts of potassium-ion concentration affect Hak1p expression only slowly and do not affect Trk1p expression at all. Time courses of Trk1p-HA (A, ■) and HA-Hak1p (B, ■) and of intracellular K⁺ () during potassium starvation (0 to 5 h), followed by potassium repletion (5 to 10 h). Removal of [K⁺]_o (harvest, rinse, and resuspend) required less than 1 min, which was very fast compared to the decline of [K⁺]_i. For strains {TRK1-HA} and {HA-HAK1}, the preparative growth medium was K⁺-free Vogel's salts plus 100 mM KCl and 2% sucrose. For the insets, the load was 5 μg membrane protein in each lane. High-sensitivity ECL reagent was used for panel A.

Inline graphic — Rapid shifts of potassium-ion concentration affect Hak1p expression only slowly and do not affect Trk1p expression at all. Time courses of Trk1p-HA (A, ■) and HA-Hak1p (B, ■) and of intracellular K⁺ () during potassium starvation (0 to 5 h), followed by potassium repletion (5 to 10 h). Removal of [K⁺]_o (harvest, rinse, and resuspend) required less than 1 min, which was very fast compared to the decline of [K⁺]_i. For strains {TRK1-HA} and {HA-HAK1}, the preparative growth medium was K⁺-free Vogel's salts plus 100 mM KCl and 2% sucrose. For the insets, the load was 5 μg membrane protein in each lane. High-sensitivity ECL reagent was used for panel A.