Table 4.
Performance parameters for GM ELISA, Aspergillus real-time PCR, and Aspergillus LFD when testing cases of proven/probable IA (n = 22) versus no IFD (n = 59)
| Parameter | Positivity thresholda | Assayb |
||
|---|---|---|---|---|
| GM | PCR | LFD | ||
| % sensitivity (95% CI) | Single | 77.27 (56.6–89.9) | 95.45 (78.2–99.2) | 81.82 (61.5–92.7) |
| Multiple | 68.18 (47.3–83.6) | 68.18 (47.3–83.6) | 59.10 (38.7–76.7) | |
| % specificity (95% CI) | Single | 81.36 (69.6–89.3) | 72.88 (60.4–82.6) | 84.75 (73.5–91.8) |
| Multiple | 91.53 (81.7–96.3) | 96.61 (88.5–99.1) | 98.00 (91.0–99.7) | |
| % PPV (95% CI) | Single | 60.71 (42.4–76.4) | 56.76 (40.9–71.3) | 66.67 (47.8–81.4) |
| Multiple | 75.00 (53.1–88.8) | 88.24 (65.7–96.7) | 93.00 (68.5–98.7) | |
| % NPV (95% CI) | Single | 90.60 (79.8–95.9) | 97.73 (88.2–99.6) | 92.59 (82.5–97.1) |
| Multiple | 88.52 (78.2–94.3) | 89.06 (79.1–94.6) | 86.57 (76.4–92.8) | |
| Positive likelihood ratio | Single | 4.15 | 3.52 | 5.37 |
| Multiple | 8.05 | 20.11 | 29.55 | |
| Negative likelihood ratio | Single | 0.28 | 0.06 | 0.21 |
| Multiple | 0.35 | 0.33 | 0.42 | |
| DOR | Single | 14.82 | 58.67 | 25.57 |
| Multiple | 23.00 | 60.94 | 70.36 | |
a
“Single” represents a threshold where the minimum requirement for patient positivity is a single positive sample per assay; “multiple” represents a threshold where two or more positive samples per patient are required for an assay to be considered positive.
b
Optimal values for each parameter are shown in bold.