Fig 1.

(A to C) Effect of metabolic stress on intracellular NPM distribution. (A) At baseline, intact cells stained with NPM antibody show prominent nucleolar staining (open arrow). In many cells, stress (ATP Deplete) induces NPM redistribution into the cytosol (closed arrow), whereas in other cells, nucleolar to nuclear NPM redistribution is detected (open arrow). During recovery, NPM reaccumulates in nucleoli (open arrow). (B) NPM redistribution into the cytosolic fraction in cells permeabilized with low-dose digitonin (upper) (see Materials and Methods) and total NPM (lower), measured by immunoblot analysis. GAPDH and β-actin were used as loading controls. (C) Densitometric analysis of four separate immunoblot studies. *, P < 0.05 versus the baseline; n = 5 separate studies. CTL, control.