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Effect of metabolic stress on NPM-Bax interaction. Immunoprecipitates (IP) were harvested from cells using an antibody directed against Bax at baseline (Base), immediately after 60 min of ATP depletion (ATP Depl), and after 30 or 60 min of recovery (Recovery) and then probed for Bax (upper) or NPM (lower) content. Immunoglobulin G (IgG) is shown as a control. Studies were performed on 400-μg cell lysate samples. IB, immunoblot.
