Fig 8.

(A and B) Effect of an NPM-Bax blocking peptide on cell survival after metabolic stress. (A) Effect of a cell-permeable NPM-Bax peptide added to the medium 1 h before, during, or after subjecting proximal tubule cells to 60 min of ATP depletion on cell survival versus no peptide or a control (CTL) peptide. *, P = 0.08 versus CTL or no siRNA peptide; n = 3 to 4 separate experiments. (B) Immunoblot analyses of cytochrome c leakage (Cytosolic cyto c), active Bax content (Bax 6A7), and active caspase 3 (active Casp 3) after stress. GAPDH and β-actin were used as loading controls.