Fig 11.

Both RIP1 and LUBAC are essential for TNF-α-induced efficient expression of a subset of NF-κB target genes. (A and B) WT and RIP1 KO MEFs were treated with mTNF-α for 2 h as indicated, and expression of IP-10, RANTES, and IL-6 was then determined by real-time RT-PCR. *, P < 0.05; **, P < 0.01. The error bars indicate SD. (C and D) RIP1 WT, KO, pBa-C, and pBa-RIP1 MEFs were treated with mTNF-α (10 ng/ml) as indicated, and expression of IP-10, IL-6, and RANTES was determined by multiplex RT-PCR.