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HuR overexpression does not affect p19ARF levels or replicative life spans. (A) Wild-type MEFs were infected with retroviruses encoding GFP (control) or HA-HuR. Infected cells were cultured by the NIH 3T3 protocol. Error bars represent SEM of results from triplicate wells. (B) Cell lysates were prepared at the indicated passage numbers. The expression of the indicated proteins was analyzed by immunoblotting. Lamin A/C was used as a loading control.
