Fig 6.
Loss of HuR relocalizes ARF mRNA to nucleoli. (A) DNA constructs for the expression of MS2-tagged ARF mRNA (full length or Δ5′UTR). CMVp, cytomegalovirus promoter. (B) Sh-SCR- or sh-HuR-expressing ARF p53 DKO cells were transiently transfected with MS2-GFP-NLS plasmids. Where indicated, cells were cotransfected with MS2-tagged ARF (full length or Δ5′UTR)-expressing plasmids. Three days later, cells were fixed in paraformaldehyde and stained with DAPI. Bars, 10 μm (C) Sh-HuR-expressing ARF p53 DKO cells were transiently transfected with MS2-GFP-NLS together with MS2-tagged ARF (full length)-expressing plasmids. Cells were stained using p19ARF antibody and DAPI.