Fig 7.

HuR weakly associates with ARF mRNA in living cells. (A) Lysates of (wild-type) MEFs expressing HA-tagged HuR proteins were immunoprecipitated using HA antibody. RNA extracted from the immune complex was analyzed by real-time PCR. Error bars represent SEM (n = 3). (B) Lysates prepared from wild-type MEFs were immunoprecipitated using control (IgG) or HuR antibodies. RNAs were extracted from immune complexes and subjected to real-time PCR analysis. p53, c-myc, and β-actin were used as positive controls. (C) Lysates were prepared from UV-cross-linked MEFs and immunoprecipitated using control or HuR antibodies. RNAs were recovered from the immune complex following proteinase K treatment and analyzed by real-time PCR for ARF mRNA levels. Error bars represent SEM of results from triplicate samples.