Fig 9.
HuR does not affect Ink4a translation. (A) NIH 3T3 cells expressing sh-SCR or sh-HuR were transfected with plasmids bearing full-length Ink4a, including its 5′- and 3′UTRs (full length), or Ink4a lacking its 5′- and 3′UTRs (ORF) together with GFP expression plasmids. Three days later, RNAs were extracted and the expression of exogenous Ink4a mRNA was analyzed by real-time PCR. Values were normalized to GFP expression levels in each sample. (B) The expression of p16Ink4a and GFP was analyzed by immunoblotting. (C) p16Ink4a levels were quantified and normalized to Ink4a expression levels. Error bars represent SEM of results from triplicate samples.