Fig 6.

Ex vivo rearrangement of Igλ genes in B220⁺ Igκ⁻ Igλ⁻ splenic cells of E3′^F/F Ed^−/− CD23-Cre mice. (Top) B220⁺ Igκ⁻ Igλ⁻ splenic B cells from E3′^F/F Ed^−/− CD23-Cre mice were isolated with high purity through FACS sorting (0 h). After culturing for 24 h in the presence of BAFF (66 ng/ml), around 40% of the cells remained viable based on their FSC and SSC patterns as assayed by FACS or 7-AAD staining (R1 components). (Middle) Surface expression of B220 of unsorted cells and in R1 gate-sorted cells (0 h) or cells cultured for 24 h was analyzed. (Bottom) Igκ and Igλ expression in R3-gated cells was assayed by FACS, and data are representative of three independent experiments.