Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Source |
|---|---|---|
| Strains | ||
| Helicobacter pylori | ||
| G27 | Clinical isolate, wild type | Laboratory collection |
| SS1 | Wild type | Laboratory collection |
| G27 CC11 | hp1043 C215S C221S (CC11) mutant of G27, Cmr | This work |
| G27 HP1043 WT Cmr | Control strain for G27 CC11, G27 Cmr | This work |
| SS1 CC11 | hp1043 C215S C221S (CC11) mutant of SS1, Cmr | This work |
| Escherichia coli | ||
| DH5α | endA1 hsdR17 glnV44 thi-1 recA1 gyrA relA1 Δ(lac-argF)U169 deoR ϕ80dlacZΔM15 | Invitrogen |
| BL21CodonPlus(DE3)-RIPL | ompT hsdS dcm+ Tetr gal (DE3) endA hte [argU ileY proL leuW Cmr Strep/Specr] | |
| Stratagene | ||
| Plasmids | ||
| pET15b | Apr T7 promoter | Novagen |
| pET15b-hp1043 | Apr hp1043, T7 promoter control | This work |
| pET15b-hp1043CC11 | Apr hp1043 CC11, T7 promoter control | This work |
| pCR2.1-TOPO | Apr Kmr | Invitrogen |
| pTOPO-hp1043pr | Derivative of pCR2.1-TOPO containing a 430-bp PCR fragment resulting from amplification of the hp1043 promoter region with oligonucleotides 104-PF and 1043-PF from the G27 genome, Apr Kmr | This work |