Fig 5.
Detecting N-acetylglucosamine (GlcNAc) in B. burgdorferi culture media and cells. The intracellular level of GlcNAc and the level of GlcNAc in culture media were measured using LC-MS/MS. (a) The intracellular concentration of GlcNAc was determined at various stages of the growth curve for BSK-II(−GlcNAc)+chitobiose medium as indicated and at the late stationary phase for cultures in BSK-II medium. Samples were measured in triplicate, and data are represented as means ± SEM in μM/108 cells. (b) Concentration of GlcNAc in normal BSK-II, GlcNAc-depleted BSK-II BSK-II(−GlcNAc) and BSK-II(−GlcNAc)+chitobiose. Asterisks indicate no GlcNAc peak detected.