Fig 1.

TLR2 and TLR6 cooperate to detect B. abortus and induce NF-κB activation. HEK 293A cells were transiently transfected with plasmids to express TLRs individually (TLR1, TLR2, or TLR6) or in combination (TLR1/TLR2 or TLR2/TLR6). These were stimulated for 24 h with viable B. abortus (MOI, 1:100), Pam₃CSK₄ (1 μg/ml), MALP2 (100 ng/ml), or L-Omp19 (500 ng/ml). The cells were disrupted with 1× lysis buffer (Promega) and assayed for firefly luciferase normalized with Renilla luciferase. Data are expressed as the mean ± standard deviation (SD). Each experiment was performed three times, and data represent one of the three experiments. Statistically significant differences relative to noninfected cells (NI) are denoted by *, relative to TLR1 by #, and relative to TLR2 by & (P < 0.05).