Fig 2.

TLR2 and TLR6 mRNAs are expressed in murine macrophages and dendritic cells after B. abortus infection. BMDMs (A) or BMDCs (B) from C57BL/6 mice were infected with B. abortus S2308 strain (MOI, 1:1000) or treated with medium alone (NI). Total RNA was extracted at 24 h postinfection, and cDNA was generated. The primary transcript levels of TLR1, TLR2, and TLR6 were determined using real-time PCR. The data are expressed as the mean ± SD of the relative levels of mRNA after normalization with β-actin mRNA expression. This experiment was performed three times, and the results are representative of one experiment with similar results. Statistically significant differences relative to noninfected cells (NI) are denoted by *, relative to TLR1 by #, and relative to TLR2 by & (P < 0.05).