Fig 1.

Isolation and identification of CPBF6-binding proteins. Lysates of CPBF6-HA, CPBF8-HA, and control (HA) transformants were mixed with anti-HA-antibody-conjugated agarose, washed, and eluted with HA peptide. The eluates were separated on SDS-PAGE and silver stained (A) or subjected to Western blotting with anti-α-amylase, anti-γ-amylase, and anti-HA antibody (B). Apparent molecular masses of standards are indicated on the left. Three bands excised for protein identification are labeled (A to C).